Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,619)

Singleplex and Multiplex Bead Based Immunoassays (6,174)

ELISA Substrates and Reagents (2,341)

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466 – 480 of 138,521 results

466

Invitrogen™ IL-5 Mouse ProcartaPlex™ Simplex Kit

The Mouse IL-5 Simplex ProcartaPlex Kit measures IL-5 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.

Pricing & Availability

467

Invitrogen™ Human TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	7.5%
Intraassay CV	4.4%
Incubation Time	4 hr.
Gene Symbol	Tnf
Total Assay Time	4 hr
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	1.7 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
Marker Gene	TNF-α
For Use With (Application)	Colorimetric Detection
Test Time	1 hr 20 min
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

468

Invitrogen™ Human Osteocalcin ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Protein Family	Hormones & Receptors
Protein Aliases	Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	4.6%
Intraassay CV	3.9%
Gene Symbol	BGLAP
Total Assay Time	2 hr 30 min
Assay Sensitivity	0.08 ng/mL
Storage Requirements	2-8°C
Assay Range	1.56-75 ng/mL
Test Time	1 hr 20 min
Accession Number	P02818
Regulatory Status	RUO
Target	Osteocalcin
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls,Standard,Standard Dilution Buffer,Incubation Buffer,HRP conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls
Gene Alias	Bglap; Bglap1; Bglap2; BGP; Bgpr; Bgpra; bone gamma carboxyglutamate protein; bone gamma carboxyglutamate protein 1; bone gamma-carboxyglutamate (gla) protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein; bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamate protein osteocalcin; bone gamma-carboxyglutamate; BGLAP; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); bone Gla protein; bone gla protein BGP; bone Gla-protein; gamma-carbooxyglutamate (gla) protein; Gamma-carboxyglutamic acid-containing protein; GLA; Gla protein precusor; mOC-A; OC; OCN; OG1; Osteocalcin; PMF1; RP11-54H19.5; unnamed protein productBglap; Bglap1; Bglap2; BGP; Bgpr; Bgpra; bone gamma carboxyglutamate protein; bone gamma carboxyglutamate protein 1; bone gamma-carboxyglutamate (gla) protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein; bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamate protein osteocalcin; bone gamma-carboxyglutamate; BGLAP; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); bone Gla protein; bone gla protein BGP; bone Gla-protein; gamma-carbooxyglutamate (gla) protein; Gamma-carboxyglutamic acid-containing protein; GLA; Gla protein precusor; mOC-A; OC; OCN; OG1; Osteocalcin; PMF1; RP11-54H19.5; unnamed protein product; BGP; OC; OCN
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	632
Sample Type	Plasma,Serum
Label or Dye	HRP

469

Invitrogen™ Human VEGF ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	4.7%
Incubation Time	4 hr.
Gene Symbol	VEGFA
Total Assay Time	4 hr
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2-8°C
Assay Range	23.4-1500 pg/mL
Marker Gene	VEGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hr 20 min
Accession Number	P15692
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

470

Invitrogen™ Human PARP (Cleaved) [214/215] ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	5.3%
Intraassay CV	4.6%
Gene Symbol	PARP1
Total Assay Time	4 hr
Assay Sensitivity	<0.062 ng/mL
Storage Requirements	2-8°C
Assay Range	0.156-10 ng/mL
Test Time	1 hr 20 min
Accession Number	P09874
Regulatory Status	RUO
Target	PARP1 (cleaved Asp214)
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; Adprt; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; Parp1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1; synthetase fragment (AA 67); Unknown (protein for IMAGE:8023736); unnamed protein product5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; Adprt; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; Parp1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1; synthetase fragment (AA 67); Unknown (protein for IMAGE:8023736); unnamed protein product; ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	142
Sample Type	Cell Lysates
Label or Dye	Biotin

471

Invitrogen™ Rat TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Content And Storage	Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

472

Promotions Available

R&D Systems™ Human IL-12 p70 Quantikine ELISA Kit

Human IL-12 p70 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human IL-12p70
Target Species	Human
Conjugate	HRP
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3592
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum,Urine
Sample Volume	Cell Culture Supernates (200uL),Serum (200uL),EDTA Plasma (200uL),Heparin Plasma (200uL),Citrate Plasma (200uL),Urine (200uL)
Synonym	CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12 A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35
Assay Sensitivity	5pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	7.80 - 500pg/mL
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

473

Invitrogen™ Mouse VEGF-A ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	6.4%
Intraassay CV	3.8%
Gene Symbol	VEGFA
Total Assay Time	4 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	20.0 pg/mL
Storage Requirements	2-8°C
Assay Range	62.5-4000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	Q00731
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	22339
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Vascular endothelial growth factor A, VPF

474

Invitrogen™ ProcartaPlex™ Mouse Cytokine & Chemokine Convenience Panel 1, 26plex

The ProcartaPlex Mouse Cytokine & Chemokine Convenience Panel 1 26plex enables the study of immune function by analyzing 26 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

475

Invitrogen™ ProcartaPlex™ Wash Buffer (10X)

ProcartaPlex Wash Buffer is for use with ProcartaPlex assays. It is supplied as a 10X concentrate and used for washing the ProcartaPlex assays using flat bottom plates.

Pricing & Availability
Specifications

For Use With (Equipment)	Luminex™ Instruments
Product Type	Buffer

476

Invitrogen™ Human Albumin ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Family	Other Proteins
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Research Discipline	Other Research
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	Human Serum Albumin
Specificity	This ELISA pair antibody detects human Albumin. Other species not determined.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	60 pg/mL to 12,000 pg/mL
Interassay CV	<12%
Gene Symbol	ALB
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	6-1200 ng/mL
Accession Number	P02768
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	213
Sample Type	Plasma,Serum,Supernatant
Synonym	GIG20, ANALBA, FDAH, PRO0883, PRO0903, PRO1341

477

Invitrogen™ Human CD163 ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Family	Other Proteins
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Assay Sensitivity	30 pg/mL
Research Discipline	Other Research
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	CD163
Specificity	In this sandwich immunoassay, no cross-reactivity with recombinant mouse CD163, rmCD6 or recombinant human CD6.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	30 pg/mL to 8000 pg/mL
Interassay CV	<12%
Gene Symbol	CD163
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	30-8000 pg/mL
Accession Number	Q86VB7
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	9332
Sample Type	Plasma,Serum,Supernatant
Synonym	M130, MM130

478

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Pricing & Availability
Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Detection Method	Colorimetric

479

Novus Biologicals™ Human Prothrombin Fragment 1+2 ELISA Kit (Colorimetric)

Colorimetric ELISA Kit

Pricing & Availability

480

Invitrogen™ Human IL-17A ProQuantum Immunoassay Kit

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Proximity-based Immunoassay
Target Species	Human
Conjugate	FAM
Incubation Time	1 hr.
Total Assay Time	2 hr
Sample Volume	Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity	<0.3 pg/mL
Storage Requirements	-20°C
Assay Range	0.32-25000 pg/mL
For Use With (Application)	Protein Quantitation
Test Time	30 min
Detection Method	Taqman™ Real-time PCR
Accession Number	Q16552
Regulatory Status	RUO
Target	IL-17A
Kit Contents	Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment)	qPCR Instruments
Product Type	ProQuantum
Gene ID (Entrez)	3605
Sample Type	Plasma,Serum,Supernatant
Label or Dye	FAM™

Immunoassay Reagents and Kits

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R&D Systems™ Human IL-12 p70 Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Novus Biologicals™ Human Prothrombin Fragment 1+2 ELISA Kit (Colorimetric) Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Invitrogen™ Human IL-17A ProQuantum Immunoassay Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

R&D Systems™ Human IL-12 p70 Quantikine ELISA Kit

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Novus Biologicals™ Human Prothrombin Fragment 1+2 ELISA Kit (Colorimetric)

Invitrogen™ Human IL-17A ProQuantum Immunoassay Kit